Genetics and Biochemistry of Class II Phosphotransacetylases

Genetics and Biochemistry of Class II Phosphotransacetylases

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Integrating metabolism and gene expression is a robust method for adapting to changes in the environment. This thesis identifies class II phosphotransacetylases as central metabolic enzymes with the ability to integrate multiple metabolic signals to alter the distribution of two central metabolites---acetyl-phosphate and acetyl-coenzyme A. The fate of these critical compounds determines the metabolic output of the microbial cell.... tungstate stain (Polysciences, Inc.) and placed onto 300 mesh Cu2+ and Ni2+ grids using PioloformAr support film. ... Cells were viewed on a Nikon Eclipse TE300 inverted microscope mated to a TE-FM epifluorescence adaptor (Nikon) andanbsp;...

Title:Genetics and Biochemistry of Class II Phosphotransacetylases
Author: Shaun Richard Brinsmade
Publisher:ProQuest - 2007

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